|
KMID : 0360220080490020319
|
|
Journal of the Korean Ophthalmological Society
2008 Volume.49 No. 2 p.319 ~ p.332
|
|
|
Inhibitory Effects of Paclitaxel and Cisplatin on Transdifferentiation of Lens Epithelial Cells into Fibroblast
|
|
Kim Lee-Hoo
Kim Dae-Hyun
Oh Jung-Hyub
|
|
|
Abstract
|
|
|
Purpose : This study investigated the inhibitory effects of Paclitaxel by altering tubulin assembly and cisplatin
exposure by binding DNA of the lens epithelial cells (LECs) during epithelial cell cultures in the capsular
bag model.
Methods : In the capsular bag model, the LECs were cultured with exposure to Paclitaxel (1, 10, 100 nM)
and Cisplatin (1, 10, 100 μM) for 3 min. The effect of Paclitaxel and Cisplatin was analyzed by observing
the cell number of fibroblasts per field, Western blots for type IV collagen, TUNEL assay and the
Proliferating Cell Nuclear Antigen (PCNA) and Bromodeoxyuridine (BrdU) incorporated proliferating cells.
Results : An increase in concentration of Paclitaxel and Cisplatin resulted in a decrease in the number of
fibroblasts and spindle-shaped cells. The number of proliferating cells showing PCNA positivity and BrdU
incorporation in the nuclei was decreased in a dose dependent manner by treatments of Paclitaxel and
Cisplatin. Expression of type IV collagen also decreased after treatment with these two agents. Results of the
TUNEL assay showed no change in the apoptosis of cells with regard to an increase in concentration of
Paclitaxel and Cisplatin.
Conclusion : This study showed inhibitory effects of Paclitaxel and Cisplatin on the proliferation and
transdifferentiation of LECs into fibroblasts using the capsular bag model.
J Korean Ophthalmol Soc 49(2):319-332, 2008
|
|
|
KEYWORD
|
|
|
Capsular bag model, Cisplatin, Fibroblast, Paclitaxel, Transdifferentiation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|